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5-10 yrs
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Laboratory & Research Specialist II
To obtain a position which would enable me to utilize my experiences in the laboratory as well as apply my Master's degree in environmental science & management
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SUPERVISOR: Dr. Robert Nakamoto 434-982-0279
*Create expression library for all open reading frames of the predicted membrane proteins of Mycobacterium tuberculosis
* Perform standard molecular biology procedures, polymerase chain reaction, DNA and protein gel electrophoresis, Western blots, fermentations, membrane protein preparations, solubilization of proteins, protein purifications
*Construct and manage Excel database for cDNA expression library
*Present research progress reports to members of consortium and laboratory
*Train new hire technicians on laboratory instruments and with techniques
*Order and maintain supplies
Poster Abstracts:
"Genomics approach to expression of membrane proteins from Mycobacterium tuberculosis" Y.B. Peskova, K.A.Rohal, R.K.Nakamoto. March 2003-Biophysical Society Abstract for 2003 convention
"Membrane Protein Structural Genomics: Mycobacterium tuberculosis" F.Gao, Y.Hua, A.Korepanova, Y.Peskova, K.Rohal, R.K.Nakamoto, T.A.Cross. March 2003-Biophysical Society Abstract for 2003 convention
August 1997-November 2001 (45+hrs/week)
Senior Microbiologist -Quality Control, HVL Incorporated/Douglas Laboratories (Pittsburgh, PA) SUPERVISOR: Dr. Naieem Shaikh 412-494-0100
Improved Microbiological Testing Methods
*Independently instituted and tested validity of cost saving tests for Salmonella sp., reducing test time from 4-5 days to 48 hours
*Improved accuracy of methods to decrease the number of retests *Increased testing efficiency by incorporating prepared media into daily testing practices, doubling daily production
*Periodically revised microbiological testing methods as found in the United States Pharmacopeia
Implemented a Laboratory Quality Assurance Program
*Wrote Standard Laboratory Procedures for the Microbiology laboratory
*Updated and improved Laboratory Training Manual by adding safety guidelines and revisions to the testing methods
*Ensure standard validations and necessary maintenance of laboratory instruments
Maintained Laboratory Efficiency
*Interviewed and trained new microbiologists
*Prepared laboratory inventory reports
*Maintained supplies for instruments and sample collection
May-June 2001
Environmental Scientist I-Michael Baker Corporation (Coraopolis, PA) SUPERVISOR: Rich Hoff 412-269-6300
*Supported the development of a combined community relations plan for two military bases
*Revised the final drafts for a Record of Decision and Site Screening Process report
*Updated ecological risk models and the corresponding Remedial Investigation report
March 5-9, 2001(Independent Study)
Auditor Trainee-Alcoa Environmental Study Program Alcoa, Inc. (Pittsburgh, PA) MENTOR: William O’Rourke 412-553-4545
*Participated as an environmental, health and safety auditor trainee with Alcoa at their IAFC facility in Auburn, Indiana
*Interviewed employees pertaining to environmental and health and safety issues, learning first-hand about the audit process
*Provided an objective evaluation to the Alcoa auditors about the strengths and weaknesses of their audit program in relation to the 'audit process' as discussed in coursework at Duquesne University
Summer 2000
Environmental Intern, Raccoon Creek State Park (Hookstown, PA) SUPERVISOR: Patrick Adams 724-899-2200
*Served as an environmental educator for the general public, hosting events such as interpretive hikes on local wildflowers and historical areas in the park
*Developed a public relations plan for Frankfort Mineral Springs: implemented surveys and free programs to solicit more public interest and to acquire more volunteers for maintenance of the area
*Created a photographic display and brochure for the park area
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