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US-Colorado-Boulder/Fort Collins
1-3 yrs
Post Doctoral Fellow
I am looking for employment that allows me to work with people I enjoy and towards goals that I am excited about. I want to complete each day thinking how lucky I am to have the opportunity to work where I do. I also must be able to respect the social and environmental ideologies of my employer.
des aegypti for Dengue 2 virus
Molecular & population genetic determinants of vector competence using SSCP analysis of RAPD and cDNA markers
Generation of mosquito lines with desired phenotype using QTL analysis and MAS
Weekly / Monthly presentations of results to advisory committee
1998 Graduate Teaching Assistant, Colorado State University, Fort Collins, CO
MB 302, a beginning laboratory in microbiology
Prepared lecture and laboratory supplies for 2 classes that each met 4 hours per week
Facilitated the student lab experience and provided a safe, educational environment
1996-1998 Project Manager, New York Blood Center, Cameroon Laboratory, Kumba, Cameroon
Supervisor: Dr. Sara Lustigman at NYBC, New York, NY
Organized & implemented onchocerciasis research project for large-scale production of Onchocerca volvulus and Onchocerca ochengi
Directed regional putative immune studies of individuals in 5 villages
Managed and trained a staff of up to 60 people in 4 departments
Designed & implemented fieldwork and laboratory protocols
Responsible for project budget; compiled monthly and annually
Facilitated research trips for visiting scientist
Generated, analyzed and presented project research results and statistics
1995-1996 Research Assistant , University of Notre Dame, Notre Dame, IN
Supervisor: Dr. George B. Craig, Jr.
Maintained 10-20 mosquito colonies in various stages of development
Collected and identified mosquito species for St. Joseph County Mosquito Abatement Program and the Eastern Equine Encephalitis surveillance program
Responsible for ordering lab materials and equipment and maintaining ongoing experiments
1995 Internship, ORSTOM at Institut Pasteur, Dakar, Senegal
Direct report to Dr. Didier Fontenille
Surveillance of anopheline prevalence & diversity in human baited catches and resting mosquito samples from living units in small villages (PCR used to distinguish sibling species of An gamibiae)
Monitored resting mosquito blood meal source using ELISA
Determined rates of malaria infection using ELISA to distinguish different Plasmodium species
PROFESSIONAL MEETINGS
1995 American Mosquito Control Association Annual Meeting, Newport, VA
Student competition presentation of anopheline mosquitoes in Senegal
2000 American Society of Virology, Ft. Collins, CO
K.E. Bennett, S. Higgs, K.E. Olson, W.C. Black, B.J. Beaty. Characterizations of infection, replication and transmission of Dengue 2 in 22 populations of Aedes aegypti from Mexico and United States.
2000 American Society of Tropical Medicine and Hygiene, Houston TX
K.E. Bennett, K.E. Olson, M.L. Munoz, I. Fernandez-Salaz, J.A. Farfan, W.C. Black IV, and B.J. Beaty. Population genetics of vector competence for Dengue 2 in the yellow fever mosquito, Aedes aegypti.
2001 American Society of Tropical Medicine and Hygiene, Atlanta, GA
K.E. Bennett, B.J. Beaty, W.C. Black IV. 2001. Quantitative trait loci for dengue susceptibility in Aedes aegypti can be used in marker assisted selection to generate mosquitoes of desired phenotype.
2002 American Mosquito Control Association, Denver, CO
K.E. Bennett, B.J. Beaty, W.C. Black IV. Quantitative trait loci for dengue susceptibility in Aedes aegypti.
2002 American Society of Tropical Medicine and Hygiene, Denver, CO
K.E. Bennett, B.J. Beaty, W.C. Black. Analysis of quantitative traits that contribute to vector competence for dengue 2 in Aedes aegypti.
2002 Entomological Society of America, Fort Lauderdale, FL
Kristine E. Bennett and W.C. Black IV. Quantitative trait loci for dengue susceptibility in Aedes aegypti.
2003 American Society of Tropical Medicine and Hygiene, Philadelphia, PA
Kristine Bennett, Barry J. Beaty, and W.C. Black IV. Identification of genes that condition dengue type-2 virus (DEN-2) susceptibility in Aedes aegypti (Diptera: Culicidae)
PUBLICATIONS
Turaga PS. Tierney TJ. Bennett KE. McCarthy MC. Simonek SC. Enyong PA. Moukatte DW. Lustigman S.. 2000. Immunity to Onchocerciasis: Cells from Putatively Immune Individuals Produce Enhanced Levels of Interleukin-5, Gamma Interferon and Granulocyte-Macrophage Colony-Stimulating Factor in Response to Onchocerca volvulus Larval and Male Worm Antigens. Infection and Immunity. 68(4):1905-1911.
Beaty BJ, Black WCIV, Olson KE, Gorrochotegui-Escalante N, Bennett KE, Bosio CF, Farfan J, Fernαndez-Salas I, Muρoz ML. 2000. Aedes aegypti and Dengue Viruses: Determination of Vector-Virus Interactions. International Meeting on Vector Borne Diseases and Environmental Relationships held in Manaus, Brazil. Pag. 1-4.
Bennett KE. Olson KE. Munoz Mde L. Fernandez-Salas I. Farfan-Ale JA. Higgs S. Black WC 4th. Beaty BJ.. 2002. Variation in Vector Competence for Dengue 2 Virus Among 24 Collections of Aedes aegypti from Mexico and the United States. Am. J. Trop. Med. Hyg. 67(1): 85-92.
Black WC 4th. Bennett KE. Gorrochotegui-Escalante N. Barillas-Mury CV. Fernandez-Salas I. de Lourdes Munoz M. Farfan-Ale JA. Olson KE. Beaty BJ. 2002. Flavivirus Susceptibility in Aedes aegypti. Archives of Medical Research. 33(4):379-88.
MacDonald AJ. Turaga PS. Harmon-Brown C. Tierney TJ. Bennett KE. McCarthy MC. Simonek SC. Enyong PA. Moukatte DW. Lustigman S.. 2002. Differential Cytokine and Antibody Responses to Adult and Larval Stages of Onchocerca volvulus Consistent with the Development of Concomitant Immunity. Infection and Immunity. 70(6): 2796-804.
Gorrochotegui-Escalante N, Lozano Fuentes S, Bennett KE, Beaty BJ, and Black WCVI. 2003. Association Mapping of Segregating Sites in the Midgut Early Trypsin Gene and Susceptibility to Dengue-2 Virus in the Mosquito Aedes aegytpi. In Review.
Bennett KE, Beaty BJ, and Black WCVI. 2003. Selection of D2S3, an Aedes aegypti Strain with High Oral Susceptibility to Dengue 2 Virus and D2MEB, a Strain with a Midgut Barrier to Dengue 2 Escape. J Med Ent. 42(2): 110-119.
Gomez-Machorro C, Bennett KE, Munoz ML, and Black WC 4th. 2004. Quantitative Trait Loci Affecting Dengue Midgut Infection Barriers in an Advanced Intercross Line of Aedes aegypti. Ins. Mol. Biol. 13(6):637-648.
Molina-Cruz A, Gupta L, Richardson J, Bennett KE, Black, WC 4th, Barillas-Mury C. 2004. Inhibition of Midgut Trypsin Activity During Blood Feeding Decreases Dengue-2 Virus Infection and Dissemination in Aedes aegypti. Am. J. Trop. Med. Hyg. In Press.
Bennett KE, Flick D, Fleming KH, Jochim, RC, Beaty BJ, and Black WC 4th. 2004. Quantitative Trait Loci that Control Dengue-2 Virus Dissemination in the Mosquito Aedes aegypti. Genetics. In Press.
COURSES
2001 Biology of Disease Vectors 11th International Course. Českι Budějovice, Czech Republic. 16-30 June, 2001.
2003 Introduction to SeqLab: Introduction to the GCG Wisconsin Package with SeqLab Interface. Centers for Disease Control and Prevention. Atlanta, GA. 11-13 February, 2003.
2004 Workshop on Molecular Evolution. Marine Biological Laboratory. Woods Hole, MA. 25 July 6 August, 2004.
LABORATORY SKILLS
PCR
Electrophoresis
SSCP
ELISA
IFA
Mosquito husbandry
Cloning
Artificial membrane feeding of mosquitoes
DNA Sequencing
Intrathoracic inoculation of mosquitoes
Mosquito dissections (midgut, salivary gland)
Slide mounting mosquito larval and pupal skins and male genitalia for morphological identification
Cell culture (mammalian and mosquito)
Virus culture (RNA viruses)
Proficiency using GENBANK and primer design programs
SOFTWARE SKILLS
PC and Macintosh platforms and software
Microsoft PowerPoint
Adobe Photoshop
Microsoft Excel / Word
SAS
GCG Wisconsin Package
Statistical and gene mapping software; i.e. Arlequin, JOINMAP
Sequence alignment/phylogeny software; i.e. PAUP, Phylip, ClustalX
QTL Cartographer, Binary QTL
DNA-Star
LINGUISTIC SKILLS
French (conversational, written and spoken)
Wolof (basic understanding, spoken African language - Senegal)
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