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Canada-Ontario
1-3 yrs
Yes
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Research Technician
A hands-on role in research that utilizes my skills and provides an opportunity for me to grow and develop in the role.
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Research Technician, Cangene Corporation, Mississauga, Ontario
•Isolated cDNA, cloning from expression libraries, DNA extractions, mammalian/ bacterial cell culture and purification of soluble monoclonal antibodies.
•Performed chromatography for purification, characterization, validation and production of recombinant proteins.
•Developed purification protocol (separations, precipitation, filtration) and followed through with analytical support work (SDS-PAGE, Western blot assays, ELISA)
•Prepared monthly reports, developed SOPs and maintained GLP compliant documentation.
Jan 2001 – Jun 2004, Project Assistant, Celestica Inc., Toronto, Ontario
•Managed resources, worked as organizational support for training workshops and as logistical support for overseas staff, reconciled and processed accounts and transfers.
•Assisted team with data collection and analysis, ensuring compliance of process change control and acting as emergency contact for worldwide IT staff.
•Operated a productive liaison between logistics and planning departments as well as external clients and media.
•Initiated, managed and negotiated invoices and contracts to obtain cost reductions – achieved 23% annual savings in North America.
Dec 1998 – Jun 2000, Admin Assistant, Higher Colleges of Technology, Dubai, UAE
•Supported departments for course-correspondence, review and control of proposals, development of electronic forms to organize purchasing and student data.
•Developed official college website, video/ audio editing for fund raising, inter-college events and educational exhibitions.
May 1997 – Nov 1998, Research Assistant, Business Aid Centre, Dubai, UAE
•Researched and communicated market trends, local, federal and ISO9001 regulations.
•Prepared marketing collateral, assisted in personnel recruitment, negotiations with clients, preparation of contracts and liaison between clients and job applicants.
Dec 1995 – Jan 1997, Research Assistant, St. George’s Hospital, NSW, Australia
•Developed research methods, acquired and analyzed raw flow cytometric data on platelet activation and T-cell antigen analysis.
•Experienced in four-color analysis of hematological malignancies and worked as liaised with geriatric donors to explain research goals and obtain blood samples.
•Presented research data on Myelodysplastic Syndrome studies at local as well as at international meetings.
Feb 1993 – Nov 1995, Research Assistant, University of Sydney, Australia
•Part-time work at the Fruit Fly Research Centre using light microscopic in situ hybridization to map gene sequences and identify homologous DNA sequences in chromosomes of related species.
•Lab work using PCR screening on blood samples to identify hypertension and obesity genes in twins. Marketed the study to doctors and clinics enlisting 150 participants over a two-month period.
•Cross-referenced and organized information for publication. Duties included filing, database development, research of virtual libraries and translation of texts.
SKILLS SUMMARY:
Lab Skills
•PCR, Southern/ Northern/ Western blots, SDS-PAGE, in situ hybridization, ELISA, cell culture, cell transformation and data analysis using PROSITE and CBS Prediction Servers.
•Liquid Chromatography (ion exchange, affinity, hydrophobic interaction, gel filtration), flow cytometry and fluorescent/ chemi-luminescent immunoassay.
Computer Skills
•Proficient software skills: MS Office (Word, Excel, PowerPoint), Dbase, DOS, BASIC, HTML, JavaScript, Adobe, FPLC Director, MS Access, Chromas, Corel Paradox 8
•Database knowledge: ENTREZ/ PUBMED, EMBL/ NCBI, BLAST/ FASTA, PROSITE
Training and Workshops
•Certificate in Workplace Hazardous Material Information Safety (WHMIS). 2004
•Certificate in St. John Ambulance Safety Oriented First Aid. 2000
•Certificate in Adult Heart saver Cardio-Pulmonary Resuscitation. American Heart Association. 1999
•Course in Interpersonal skills, Teamwork and Effective Meetings. Southern Sydney Area Health Service. 1996
PRESENTATIONS AND PUBLICATIONS:
•Kunjukutty R. Update on financing for biotech. Michener 222; Oct 2004
•Roeth PJ, Kunjukutty R, Peters L and Kwon YL. Flow cytometric monitoring of thrombin activated platelet surface antigen changes. Australasian Flow Cytometry Group meeting (AFCG), Australia; Nov 1996
•Roeth PJ, Kunjukutty R, Peters L and Kwon YL. Study of expression of platelet surface antigens in MDS patients using flow cytometry. AFCG, Australia; Nov 1996
References will be available on request.
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